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An average count of 42 cells/mm2 grid is counted in a hemacytometer for a 2.5 ml suspension of cells. (Show your calculations!)

a. What is the cell concentration of the suspension?

b. Approximately how many cells are in the entire sample?

c. To perform an experiment, 8 dishes of cells with 1.2 x 105 cells per dish are needed. How many ml of the suspension will be needed for the experiment? Are there enough cells?

d. How many cells would have to be actually added to a plate (this is usually called “seeded”) to begin a culture with 3.5 x 105 cells/dish, if the line of cells is known to have a plating efficiency of 65%? (Show your calculations!)